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    NBT/BCIP Substrate Solution(Single Solution)

    更新:2021-11-12 16:52:43

    中文名:NBT/BCIP Substrate Solution(Single Solution)說明書下載暫無
    英文名:NBT/BCIP 底物溶液(單溶液)
    描述:Thermo Scientific Pierce NBT 和 BCIP 底物是用于發色印跡和 IHC 染色(使用堿性磷酸酶探針)的硝基四氮唑藍和 5-溴-4-氯-3'-吲哚基磷酸鹽粉末和即用型溶液。
    訂購信息
      貨號規格價格品牌
      E1162-100ml100ml330GBCBIO
      E1162-500ml500ml1380GBCBIO
      E1162-100ml*10100ml*102850GBCBIO
    產品介紹

      Application

      A ready-to-use, precipitating substrate system for alkaline phosphatase. This substrate system produces an insoluble NBT diformazan end product that is blue to purple in color and can be observed visually. Immunohistochemical staining with BCIP/NBT utilizes requires organic mounting media and can be counterstained with Nuclear Fast Red or Light Green. BCIP/NBT is generally a more sensitive method than Fast Red. Not recommended for ELISA (microwell) procedures.
      BCIP®/NBT Liquid Substrate System has been used:
      • for staining alkaline phosphatase(ALP) in bone marrow stromal cells
      • as a substrate in western blot detection of matrix metalloproteinase-2 specific antibody
      • as a substrate for streptavidin-alkaline phosphatase in the enzyme-linked immunospot (ELISpot) assays

      NBT(氯化硝基四氮唑藍)和 BCIP(5-溴-4-氯-3'-吲哚基磷酸甲苯胺鹽)在與堿性磷酸酶(一種常用的抗體探針酶偶聯物)反應時產生一種不溶性深黑紫色沉淀物。 NBT 和 BCIP 粉末以兩種便利、即用型溶液的形式單獨提供(含和不含內源性磷酸酶活性的左旋咪唑抑制劑)。

      特點:
      AP 底物—用于檢測固體培養基上的堿性磷酸酶 (alk-phos) 活性,包括硝酸纖維素和 PVDF 膜及固定的組織樣品
      • 發色—無需使用特殊設備即可進行可視化;產生易于拍照的紫黑色沉淀物

      NBT/BCIP 單溶液的產品優勢:
      • 適用于免疫印跡應用
      • 即用型單一組分
      • 在低背景下具有敏感性并能最小化測定間差異
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